Hotspot

tRNA-Ser(GCU)-tRNA-Ser (CGA)

LSC · 8893-10482 · 1590 bp

Back to Syngonium

Pi 0.00651

MarkerSeek score 57.80

Primer available yes

Label tRNA-Ser(GCU)-tRNA-Ser (CGA)

Feature Metadata

label_name: tRNA-Ser(GCU)-tRNA-Ser (CGA)
feature_type: igs
region: LSC
length_bp: 1590
variable_sites: 10
pi: 0.00651
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p1	GAGAAAGCGGAGAAAGAGGG	AGGACGAATCACACTTTTACCA	55.0	40.9	58.0	57.7	1722-1744 bp	1.0	64.0
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p2	ATTAGCAATCCGCCGCTTTA	AGGACGAATCACACTTTTACCA	45.0	40.9	58.0	57.7	1667-1689 bp	1.0	63.9
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p3	GAGAAAGCGGAGAAAGAGGG	GGACGAATCACACTTTTACCAC	55.0	45.5	58.0	57.6	1721-1743 bp	1.0	63.6
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p4	ATTAGCAATCCGCCGCTTTA	GGACGAATCACACTTTTACCAC	45.0	45.5	58.0	57.6	1666-1688 bp	1.0	63.5
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p5	AGAAAGCGGAGAAAGAGGGA	AGGACGAATCACACTTTTACCA	50.0	40.9	58.4	57.7	1721-1743 bp	1.0	61.3