Hotspot

tRNA-GUG-psbA

LSC · 95-518 · 424 bp

Back to Syngonium

Pi 0.033

MarkerSeek score 74.10

Primer available yes

Label tRNA-GUG-psbA

Feature Metadata

label_name: tRNA-GUG-psbA
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 14
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-GUG-psbA_p1	ATATAATGGGCGAACGACGG	GGCTGATATCATCAACCGGG	50.0	55.0	57.9	58.2	618 bp	1.0	79.7
tRNA-GUG-psbA_p2	ATATAATGGGCGAACGACGG	GGGCTGATATCATCAACCGG	50.0	55.0	57.9	58.2	619 bp	1.0	79.7
tRNA-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GGCTGATATCATCAACCGGG	55.0	55.0	58.1	58.2	557 bp	1.0	79.5
tRNA-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GGGCTGATATCATCAACCGG	55.0	55.0	58.1	58.2	558 bp	1.0	79.5
tRNA-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GGCTGATATCATCAACCGGG	50.0	55.0	57.9	58.2	571 bp	1.0	79.4