Hotspot

ycf1

SSC · 129885-135365 · 5481 bp

Back to Syndiclis

Pi 0.00289

MarkerSeek score 43.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 23
pi: 0.00289
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAGGTTTGATATGGGACGA	GACCGATATGGATACCGGGA	50.0	55.0	57.0	58.2	194 bp	1.0	72.0
ycf1_p2	TCCAGGTTTGATATGGGACG	GACCGATATGGATACCGGGA	50.0	55.0	57.0	58.2	195 bp	1.0	72.0
ycf1_p3	CCAGGTTTGATATGGGACGA	TATGGATACCGGGACCAACA	50.0	50.0	57.0	57.8	188 bp	1.0	71.9
ycf1_p4	TCCAGGTTTGATATGGGACG	TATGGATACCGGGACCAACA	50.0	50.0	57.0	57.8	189 bp	1.0	71.8
ycf1_p5	CCAGGTTTGATATGGGACGA	ATTGAGGCCTGGACCGATAT	50.0	50.0	57.0	58.3	205 bp	1.0	71.0