Hotspot

atpA

LSC · 12194-13717 · 1524 bp

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Pi 0.000656

MarkerSeek score 36.50

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 2
pi: 0.000656
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	TTTTTGTCGGGTCGGAAGAG	50.0	50.0	58.0	58.1	2594-2596 bp	1.0	79.2
atpA_p2	AGCCTTCCAAGCTAACGATG	AAGGAGCCCTAGGAACTCTG	50.0	55.0	58.0	58.1	2063-2065 bp	1.0	79.2
atpA_p3	AGCCTTCCAAGCTAACGATG	CAAGGAGCCCTAGGAACTCT	50.0	55.0	58.0	58.1	2064-2066 bp	1.0	79.2
atpA_p4	AGCCTTCCAAGCTAACGATG	CTGGGGGCCATGAAAGAAAT	50.0	50.0	58.0	58.1	1986-1988 bp	1.0	79.2
atpA_p5	AGCCTTCCAAGCTAACGATG	GGAGCCCTAGGAACTCTGAA	50.0	55.0	58.0	58.1	2061-2063 bp	1.0	79.2