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psbE-petL

LSC · 69789-71025 · 1237 bp

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Pi 0.00472

MarkerSeek score 62.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 10
pi: 0.00472
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1381-1409 bp	1.0	76.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1379-1407 bp	1.0	76.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1390-1418 bp	1.0	75.6
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.2	58.5	1388-1416 bp	1.0	75.5
psbE-petL_p5	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1395-1423 bp	1.0	75.5