Hotspot

petA-psbJ

LSC · 67866-69013 · 1148 bp

Back to Syndiclis

Pi 0.00798

MarkerSeek score 64.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 18
pi: 0.00798
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1284-1288 bp	1.0	72.8
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	45.0	50.0	58.2	56.7	1333-1337 bp	1.0	69.7
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	45.0	50.0	58.2	56.7	1332-1336 bp	1.0	69.7
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	45.0	47.6	58.2	57.5	1291-1295 bp	1.0	67.9
petA-psbJ_p5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	45.0	47.6	58.2	58.5	1333-1337 bp	1.0	67.6