Hotspot

ndhC-trnV-UAC

LSC · 54058-55895 · 1838 bp

Back to Syndiclis

Pi 0.00218

MarkerSeek score 59.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1838
variable_sites: 8
pi: 0.00218
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTTCACTAATCGGGGCCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1980-1986 bp	1.0	77.8
ndhC-trnV-UAC_p2	ACTAATCGGGGCCAAAACTC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1976-1982 bp	1.0	77.8
ndhC-trnV-UAC_p3	CACTAATCGGGGCCAAAACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1977-1983 bp	1.0	77.4
ndhC-trnV-UAC_p4	TCACTAATCGGGGCCAAAAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1978-1984 bp	1.0	77.4
ndhC-trnV-UAC_p5	CTTCACTAATCGGGGCCAAA	ACGCATGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	2046-2052 bp	1.0	76.8