Hotspot

trnE-UUC-trnT-GGU

LSC · 34258-34991 · 734 bp

Back to Syndiclis

Pi 0.00204

MarkerSeek score 60.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 3
pi: 0.00204
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATGGTCAACTCCCTTTCGT	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	883-884 bp	1.0	78.8
trnE-UUC-trnT-GGU_p2	CATGGTCAACTCCCTTTCGT	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	862-863 bp	1.0	78.4
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	836-837 bp	1.0	78.2
trnE-UUC-trnT-GGU_p4	CATGGTCAACTCCCTTTCGT	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	859-860 bp	1.0	78.2
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	815-816 bp	1.0	78.1