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psbM-trnD-GUC

LSC · 32393-33526 · 1134 bp

Back to Syndiclis

Pi 0.00323

MarkerSeek score 61.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 7
pi: 0.00323
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	CTGTACACCTCATTTCCCCG	45.0	55.0	58.3	58.0	1271-1272 bp	1.0	78.2
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	CTGTACACCTCATTTCCCCG	45.0	55.0	57.6	58.0	1270-1271 bp	1.0	77.2
psbM-trnD-GUC_p3	AATGAACAGCGCAGTAGCAA	CTGTACACCTCATTTCCCCG	45.0	55.0	58.5	58.0	1272-1273 bp	1.0	76.4
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	GCTGTACACCTCATTTCCCC	45.0	55.0	58.3	58.2	1272-1273 bp	1.0	76.4
psbM-trnD-GUC_p5	ATGAACAGCGCAGTAGCAAT	TCCCCGGGATTGTAGTTCAA	45.0	50.0	58.3	58.3	1257-1258 bp	1.0	75.7