Hotspot

atpH-atpI

LSC · 15763-16683 · 921 bp

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Pi 0.00453

MarkerSeek score 62.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 8
pi: 0.00453
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.1	57.9	1053-1058 bp	1.0	79.1
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	1006-1011 bp	1.0	79.1
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	1111-1116 bp	1.0	79.0
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	1064-1069 bp	1.0	79.0
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	1071-1076 bp	1.0	78.8