Hotspot

atpA-atpF

LSC · 13718-13787 · 70 bp

Back to Syndiclis

Pi 0.0643

MarkerSeek score 79.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 9
pi: 0.0643
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCCACTTGAAGGACTGTTCC	CTGGGGGCCATGAAAGAAAT	55.0	50.0	58.1	58.1	206-207 bp	1.0	82.0
atpA-atpF_p2	CCCACTTGAAGGACTGTTCC	AAGGAGCCCTAGGAACTCTG	55.0	55.0	58.1	58.1	283-284 bp	1.0	81.0
atpA-atpF_p3	CCCACTTGAAGGACTGTTCC	CAAGGAGCCCTAGGAACTCT	55.0	55.0	58.1	58.1	284-285 bp	1.0	81.0
atpA-atpF_p4	CCCACTTGAAGGACTGTTCC	GGAGCCCTAGGAACTCTGAA	55.0	55.0	58.1	58.1	281-282 bp	1.0	81.0
atpA-atpF_p5	CCCACTTGAAGGACTGTTCC	TAATATTGGCATGCTGGGGG	55.0	50.0	58.1	57.7	219-220 bp	1.0	80.4