Hotspot

rps16-trnQ-UUG

LSC · 7877-8866 · 990 bp

Back to Syndiclis

Pi 0.00303

MarkerSeek score 61.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 990
variable_sites: 6
pi: 0.00303
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCAAGAATCCACAGCCGATA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1091-1101 bp	1.0	79.9
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1062-1072 bp	1.0	79.2
rps16-trnQ-UUG_p3	GCAAGAATCCACAGCCGATA	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.0	57.8	1095-1105 bp	1.0	78.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1066-1076 bp	1.0	78.1
rps16-trnQ-UUG_p5	CCACAGCCGATAATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1083-1093 bp	1.0	77.6