Hotspot

ycf37

Genome · 86323-86826 · 504 bp

Back to Synarthrophyton

Pi 0.284

MarkerSeek score 79.90

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 504
variable_sites: 139
pi: 0.284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	TCGGGATATAGCGCAGTTTG	AATCCTCTTGAAGCTCGTGG	50.0	50.0	57.9	57.6	1306-1466 bp	1.0	85.8
ycf37_p2	GTCGGGATATAGCGCAGTTT	AATCCTCTTGAAGCTCGTGG	50.0	50.0	57.8	57.6	1307 bp	0.5	55.7
ycf37_p3	TACTGTCGGTTGGGTACGTA	AATCCTCTTGAAGCTCGTGG	50.0	50.0	57.8	57.6	1715 bp	0.5	55.3
ycf37_p4	TCGGGATATAGCGCAGTTTG	ATCCTCTTGAAGCTCGTGGA	50.0	50.0	57.9	58.4	1305 bp	0.5	55.3
ycf37_p5	GTCGGGATATAGCGCAGTTT	ATCCTCTTGAAGCTCGTGGA	50.0	50.0	57.8	58.4	1306 bp	0.5	55.3