Hotspot

syfB

Genome · 104709-106703 · 1995 bp

Back to Synarthrophyton

Pi 0.301

MarkerSeek score 78.70

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 1995
variable_sites: 596
pi: 0.301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	AATCCTCCCACCCCAGTTTA	AGTAAGGGTGCAAAGGTGTG	50.0	50.0	57.9	58.0	2750-2802 bp	1.0	87.7
syfB_p2	AATCCTCCCACCCCAGTTTA	GGTGCAAAGGTGTGGTAAGA	50.0	50.0	57.9	58.0	2744-2796 bp	1.0	87.7
syfB_p3	GGGTGTAGCCAAGTGGTAAG	AGTAAGGGTGCAAAGGTGTG	55.0	50.0	57.9	58.0	2804-2856 bp	1.0	87.4
syfB_p4	GGTGTAGCCAAGTGGTAAGG	AGTAAGGGTGCAAAGGTGTG	55.0	50.0	57.9	58.0	2803-2855 bp	1.0	87.4
syfB_p5	TATCAACGGTCAGCCCAAAG	AGTAAGGGTGCAAAGGTGTG	50.0	50.0	57.9	58.0	2398 bp	0.5	59.0