Hotspot

ycf4

LSC · 62245-62799 · 555 bp

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Pi 0.00772

MarkerSeek score 44.10

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 10
pi: 0.00772
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TCCAATAAAACGCAATCAGATCA	CTCCTGATGAGAAAGCATTCA	34.8	42.9	56.9	55.7	618 bp	1.0	41.3
ycf4_p2	AAAACGCAATCAGATCAAGT	CTCCTGATGAGAAAGCATTCA	35.0	42.9	53.8	55.7	612 bp	1.0	41.3
ycf4_p3	TCTCTCAATTCCAATAAAACGCA	CTCCTGATGAGAAAGCATTCA	34.8	42.9	56.8	55.7	627 bp	1.0	41.3
ycf4_p4	TGGCTATTCTCTCAATTCCA	CTCCTGATGAGAAAGCATTCA	40.0	42.9	53.7	55.7	634 bp	1.0	41.3
ycf4_p5	TGGCTATTCTCTCAATTCCAA	CTCCTGATGAGAAAGCATTCA	38.1	42.9	54.6	55.7	634 bp	1.0	41.3