Hotspot

rpl20

LSC · 71271-71624 · 354 bp

Back to Symplocos

Pi 0.00726

MarkerSeek score 31.90

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 354
variable_sites: 6
pi: 0.00726
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	TTGAAAGAACCGAGTCGACC	AGAACGGACTAACAGGGTCA	50.0	50.0	57.9	58.0	1172-1181 bp	1.0	80.2
rpl20_p2	TTGAAAGAACCGAGTCGACC	GACCTCGCCGTTTAAGAAGT	50.0	50.0	57.9	57.9	988-997 bp	1.0	79.8
rpl20_p3	ACTCTACCTTCCCGGAGTTC	AGAACGGACTAACAGGGTCA	55.0	50.0	58.2	58.0	907 bp	1.0	79.6
rpl20_p4	GACTCTACCTTCCCGGAGTT	AGAACGGACTAACAGGGTCA	55.0	50.0	58.2	58.0	908 bp	1.0	79.6
rpl20_p5	CTCTACCTTCCCGGAGTTCA	AGAACGGACTAACAGGGTCA	55.0	50.0	58.2	58.0	906 bp	1.0	79.6