Hotspot

ccsA-ndhD

SSC · 118022-118255 · 234 bp

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Pi 0.0122

MarkerSeek score 63.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 234
variable_sites: 6
pi: 0.0122
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCTGCAATTGTGGCTTCT	ACCCCGATTTCGTTCTTTCA	40.0	45.0	55.9	57.4	431-448 bp	1.0	60.8
ccsA-ndhD_p2	TGCAATTGTGGCTTCTATAGGT	ACCCCGATTTCGTTCTTTCA	40.9	45.0	57.8	57.4	427-444 bp	1.0	59.7
ccsA-ndhD_p3	AATTCTGCAATTGTGGCTTCT	ACCCCGATTTCGTTCTTTCA	38.1	45.0	56.7	57.4	432-449 bp	1.0	59.0
ccsA-ndhD_p4	CAAATTCTGCAATTGTGGCT	ACCCCGATTTCGTTCTTTCA	40.0	45.0	55.4	57.4	434-451 bp	1.0	56.8
ccsA-ndhD_p5	AAATTCTGCAATTGTGGCTTCT	ACCCCGATTTCGTTCTTTCA	36.4	45.0	57.4	57.4	433-450 bp	1.0	56.7