Hotspot

trnF-GAA-ndhJ

LSC · 50433-51131 · 699 bp

Back to Symplocos

Pi 0.0108

MarkerSeek score 58.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 16
pi: 0.0108
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	841-845 bp	1.0	80.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	805-809 bp	1.0	80.5
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	850-854 bp	1.0	80.1
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	876-880 bp	1.0	79.9
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	829-833 bp	1.0	79.8