Hotspot

trnS-GCU-trnG-GCC

LSC · 9040-9707 · 668 bp

Back to Symplocos

Pi 0.00984
MarkerSeek score 66.50
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
668
variable_sites
14
pi
0.00984
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 ATTAGCAATCCGCCGCTTTA AACCGAAAGACCCTTTAACT 45.0 40.0 58.0 54.5 777-781 bp 1.0 53.8
trnS-GCU-trnG-GCC_p2 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 739-743 bp 1.0 53.8
trnS-GCU-trnG-GCC_p3 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 742-746 bp 1.0 52.7
trnS-GCU-trnG-GCC_p4 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 741-745 bp 1.0 52.7
trnS-GCU-trnG-GCC_p5 ATTAGCAATCCGCCGCTTTA AAACCGAAAGACCCTTTAACT 45.0 38.1 58.0 55.3 778-782 bp 1.0 52.6