Hotspot

ycf4

LSC · 61570-62124 · 555 bp

Back to Symplocarpus

Pi 0

MarkerSeek score 39.10

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	GGGAGCGGGAAAAGAAAAGT	CGAATTCCTTCCCCCATGTT	50.0	50.0	58.4	57.9	675 bp	1.0	75.8
ycf4_p2	GGGAGCGGGAAAAGAAAAGT	TCCTTCCCCCATGTTGAGAA	50.0	50.0	58.4	58.2	670 bp	1.0	75.0
ycf4_p3	GGGAGCGGGAAAAGAAAAGT	TTCCTTCCCCCATGTTGAGA	50.0	50.0	58.4	58.2	671 bp	1.0	75.0
ycf4_p4	GGGAGCGGGAAAAGAAAAGT	AAAAGGGACAAGGGGGATTG	50.0	50.0	58.4	57.7	696 bp	1.0	74.3
ycf4_p5	GGGAGCGGGAAAAGAAAAGT	CCTTCCCCCATGTTGAGAAA	50.0	50.0	58.4	57.4	669 bp	1.0	72.1