Hotspot

rps16

LSC · 4916-6241 · 1326 bp

Back to Symplocarpus

Pi 0.00307

MarkerSeek score 62.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1326
variable_sites: 4
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCAAGATTAACCCCACTTTTGT	TGTACCGATTGAGCCAATGA	40.9	45.0	57.4	56.9	1503-1519 bp	1.0	52.0
rps16_p2	GCAAGATTAACCCCACTTTTGT	GTACCGATTGAGCCAATGACT	40.9	47.6	57.4	58.1	1502-1518 bp	1.0	51.8
rps16_p3	AGGCAAATGTTGAGGAAAGA	TGTACCGATTGAGCCAATGA	40.0	45.0	55.1	56.9	1425-1441 bp	1.0	50.0
rps16_p4	AGCAAGATTAACCCCACTTTTG	TGTACCGATTGAGCCAATGA	40.9	45.0	57.1	56.9	1504-1520 bp	1.0	49.8
rps16_p5	AGGCAAATGTTGAGGAAAGA	GTACCGATTGAGCCAATGACT	40.0	47.6	55.1	58.1	1424-1440 bp	1.0	49.8