Hotspot

rpl22

LSC · 85646-86035 · 390 bp

Back to Symplocarpus

Pi 0.00769

MarkerSeek score 48.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 390
variable_sites: 3
pi: 0.00769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	GGTGCTCATCATCCATTGGT	47.6	50.0	58.1	57.9	526 bp	1.0	71.4
rpl22_p2	CCAAGTCTGAAACCAAGTGGA	TCCATTGGTAGGTAAGGGGG	47.6	55.0	58.1	58.1	515 bp	1.0	71.1
rpl22_p3	ACCAAGTCTGAAACCAAGTGG	GGTGCTCATCATCCATTGGT	47.6	50.0	58.3	57.9	527 bp	1.0	69.2
rpl22_p4	CCAAGTCTGAAACCAAGTGGA	TGAATGGGTGCTCATCATCC	47.6	50.0	58.1	57.6	532 bp	1.0	69.1
rpl22_p5	ACCAAGTCTGAAACCAAGTGG	TCCATTGGTAGGTAAGGGGG	47.6	55.0	58.3	58.1	516 bp	1.0	68.9