Hotspot

rpl22-rps19

LSC · 86036-86112 · 77 bp

Back to Symplocarpus

Pi 0.013

MarkerSeek score 60.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 77
variable_sites: 1
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGCCCTCGAATCTGATCAAT	ACAGATCGTATGGTGGGTCA	50.0	50.0	57.8	58.1	277 bp	1.0	77.9
rpl22-rps19_p2	CGCCCTCGAATCTGATCAAT	AGATCGTATGGTGGGTCACA	50.0	50.0	57.8	58.1	275 bp	1.0	77.9
rpl22-rps19_p3	CATAGGAACGCCCTCGAATC	ACAGATCGTATGGTGGGTCA	55.0	50.0	58.2	58.1	285 bp	1.0	77.4
rpl22-rps19_p4	CATAGGAACGCCCTCGAATC	AGATCGTATGGTGGGTCACA	55.0	50.0	58.2	58.1	283 bp	1.0	77.4
rpl22-rps19_p5	CGCCCTCGAATCTGATCAAT	CAGATCGTATGGTGGGTCAC	50.0	55.0	57.8	57.8	276 bp	1.0	77.2