Hotspot

ycf4-cemA

LSC · 62125-63309 · 1185 bp

Back to Symplocarpus

Pi 0.00338

MarkerSeek score 60.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 1185
variable_sites: 4
pi: 0.00338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGTGCGT	TATGTGTCTCGGGGTACTCC	45.0	55.0	58.1	57.9	1252 bp	1.0	79.1
ycf4-cemA_p2	ACAAAAAGCTGCGGAATTGG	TATGTGTCTCGGGGTACTCC	45.0	55.0	57.8	57.9	1268 bp	1.0	78.6
ycf4-cemA_p3	TTGGCCTATTTCTTGTGCGT	TCGGGGTACTCCTTTAGCAT	45.0	50.0	58.1	57.8	1244 bp	1.0	78.3
ycf4-cemA_p4	TTGGCCTATTTCTTGTGCGT	TCTCGGGGTACTCCTTTAGC	45.0	55.0	58.1	58.2	1246 bp	1.0	77.8
ycf4-cemA_p5	ACAAAAAGCTGCGGAATTGG	TCGGGGTACTCCTTTAGCAT	45.0	50.0	57.8	57.8	1260 bp	1.0	77.8