Hotspot

rbcL-accD

LSC · 57935-58734 · 800 bp

Back to Symplocarpus

Pi 0

MarkerSeek score 60.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	TAGCCCACACCTGTGTTCTA	50.0	50.0	57.9	58.0	917-932 bp	1.0	80.2
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	TTTAGCCCACACCTGTGTTC	50.0	50.0	57.9	58.0	919-934 bp	1.0	80.2
rbcL-accD_p3	TAGATCCGGACCCAGTGAAA	TAGCCCACACCTGTGTTCTA	50.0	50.0	57.8	58.0	871-886 bp	1.0	79.3
rbcL-accD_p4	TAGATCCGGACCCAGTGAAA	TTTAGCCCACACCTGTGTTC	50.0	50.0	57.8	58.0	873-888 bp	1.0	79.2
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	GGGGTCTTCATTTCCACTGG	50.0	55.0	57.9	58.2	974-989 bp	1.0	79.0