Hotspot

psbM-trnD-GUC

LSC · 31026-32026 · 1001 bp

Back to Symplocarpus

Pi 0.002
MarkerSeek score 59.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1001
variable_sites
2
pi
0.002
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC AGTTCAATCGGTCAGAGCAC 45.0 50.0 55.8 57.9 1115-1233 bp 1.0 61.6
psbM-trnD-GUC_p2 AGGAACTAGAATGAACAGTGCA AGTTCAATCGGTCAGAGCAC 40.9 50.0 57.4 57.9 1122-1240 bp 1.0 58.5
psbM-trnD-GUC_p3 GGAACTAGAATGAACAGTGCAG AGTTCAATCGGTCAGAGCAC 45.5 50.0 57.1 57.9 1121-1239 bp 1.0 56.0
psbM-trnD-GUC_p4 AGAATGAACAGTGCAGTAGC GTTCAATCGGTCAGAGCACC 45.0 55.0 55.8 58.9 1114-1232 bp 1.0 54.9
psbM-trnD-GUC_p5 GGAACTAGAATGAACAGTGCA AGTTCAATCGGTCAGAGCAC 42.9 50.0 55.9 57.9 1121-1239 bp 1.0 54.7