Hotspot

ycf1

SSC · 118298-123688 · 5391 bp

Back to Symphytum

Pi 0.0123

MarkerSeek score 58.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5391
variable_sites: 66
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTTGTGCCCAGTTCTCGT	TTATCGAACCCACTCGTGTG	45.0	50.0	58.0	57.6	338 bp	1.0	78.9
ycf1_p2	ATTTTGTGCCCAGTTCTCGT	CCCACTCGTGTGTCTGTAAA	45.0	50.0	58.0	57.5	330 bp	1.0	77.6
ycf1_p3	AGTTTTCAACCGCGGATACA	TTATCGAACCCACTCGTGTG	45.0	50.0	57.8	57.6	494 bp	1.0	77.1
ycf1_p4	TTTTGTGCCCAGTTCTCGTT	TTATCGAACCCACTCGTGTG	45.0	50.0	58.2	57.6	337 bp	1.0	77.1
ycf1_p5	ATTTTGTGCCCAGTTCTCGT	ACCCACTCGTGTGTCTGTAA	45.0	50.0	58.0	58.6	331 bp	1.0	77.0