Hotspot

rps16

LSC · 4885-5990 · 1106 bp

Back to Symphytum

Pi 0.0109

MarkerSeek score 54.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1106
variable_sites: 12
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCCCCTTAATTTGTTTGTATTTCCT	ATCAACTCCACACCCCTTCT	36.0	50.0	57.7	58.3	1173-1181 bp	1.0	41.4
rps16_p2	CCCCCTTAATTTGTTTGTATTTCCT	TTGAATCAACTCCACACCCC	36.0	50.0	57.7	57.7	1177-1185 bp	1.0	41.4
rps16_p3	CCCCCTTAATTTGTTTGTATTTCCT	TCAACTCCACACCCCTTCTA	36.0	50.0	57.7	57.6	1172-1180 bp	1.0	41.4
rps16_p4	CCCCCTTAATTTGTTTGTATTTCC	ATCAACTCCACACCCCTTCT	37.5	50.0	56.5	58.3	1173-1181 bp	1.0	41.4
rps16_p5	CCCCCTTAATTTGTTTGTATTTCCT	TGAATCAACTCCACACCCCT	36.0	50.0	57.7	58.6	1176-1184 bp	1.0	41.4