Hotspot

rps15

SSC · 117704-117967 · 264 bp

Back to Symphytum

Pi 0.00379

MarkerSeek score 40.30

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 1
pi: 0.00379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	ACGAGAACTGGGCACAAAAT	45.0	45.0	58.1	58.0	926-957 bp	1.0	82.9
rps15_p2	ACCATGCATTGATGGGTGTT	ACGAGAACTGGGCACAAAAT	45.0	45.0	58.1	58.0	923-954 bp	1.0	82.9
rps15_p3	GGTCTGTGCCCCAATATCTG	ACGAGAACTGGGCACAAAAT	55.0	45.0	58.0	58.0	1238-1269 bp	1.0	82.2
rps15_p4	CACCAATAATGCCTACGCCT	ACGAGAACTGGGCACAAAAT	50.0	45.0	58.0	58.0	1482-1513 bp	1.0	81.8
rps15_p5	TCCACCAATAATGCCTACGC	ACGAGAACTGGGCACAAAAT	50.0	45.0	58.0	58.0	1484-1515 bp	1.0	81.8