Hotspot

ccsA

SSC · 109133-110104 · 972 bp

Back to Symphytum

Pi 0.00823

MarkerSeek score 50.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 8
pi: 0.00823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTTGAAGACAAGCCGCTA	TAGCTTTTCGATGGCTTCCC	50.0	50.0	57.9	58.0	1707-1718 bp	1.0	80.1
ccsA_p2	TAGACACGCTGCTCTTAGGA	TAGCTTTTCGATGGCTTCCC	50.0	50.0	57.9	58.0	1675-1686 bp	1.0	79.8
ccsA_p3	ACAAGCCGCTATGGTGAAAT	TAGCTTTTCGATGGCTTCCC	45.0	50.0	57.9	58.0	1698-1709 bp	1.0	79.7
ccsA_p4	AAATTGGTAGACACGCTGCT	TAGCTTTTCGATGGCTTCCC	45.0	50.0	57.8	58.0	1682-1693 bp	1.0	79.3
ccsA_p5	GTAGACACGCTGCTCTTAGG	TAGCTTTTCGATGGCTTCCC	55.0	50.0	57.8	58.0	1676-1687 bp	1.0	79.2