Hotspot

trnI-GAU-rrn16

IRa · 130471-130759 · 289 bp

Back to Symphytum

Pi 0.0311

MarkerSeek score 70.40

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: IRa
length_bp: 289
variable_sites: 9
pi: 0.0311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	415-420 bp	1.0	78.0
trnI-GAU-rrn16_p2	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	383-388 bp	1.0	78.0
trnI-GAU-rrn16_p3	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	55.0	50.0	57.8	58.3	437-442 bp	1.0	77.9
trnI-GAU-rrn16_p4	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.2	58.3	405-410 bp	1.0	77.8
trnI-GAU-rrn16_p5	CTATCCATGTGGCTGAGAGC	AAGTCGTAACAAGGTAGCCG	55.0	50.0	57.8	57.6	424-429 bp	1.0	77.5