Hotspot

rps15-ycf1

SSC · 117968-118297 · 330 bp

Back to Symphytum

Pi 0.0301
MarkerSeek score 73.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
330
variable_sites
9
pi
0.0301
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACAGACCCTTTGTTTTCT GGATCTGTATGTATCCGCGG 38.1 55.0 55.5 57.7 408-439 bp 1.0 55.8
rps15-ycf1_p2 TCAACAGACCCTTTGTTTTCTTC GGATCTGTATGTATCCGCGG 39.1 55.0 57.4 57.7 408-439 bp 1.0 54.9
rps15-ycf1_p3 ACAGACCCTTTGTTTTCTTCTTG GGATCTGTATGTATCCGCGG 39.1 55.0 57.4 57.7 405-436 bp 1.0 54.8
rps15-ycf1_p4 CAACAGACCCTTTGTTTTCTTCT GGATCTGTATGTATCCGCGG 39.1 55.0 57.4 57.7 407-438 bp 1.0 54.8
rps15-ycf1_p5 TGAAATTCAACAGACCCTTTGT GGATCTGTATGTATCCGCGG 36.4 55.0 56.4 57.7 414-445 bp 1.0 54.4