Hotspot

ccsA-ndhD

SSC · 110105-110277 · 173 bp

Back to Symphytum

Pi 0.0578

MarkerSeek score 72.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 173
variable_sites: 10
pi: 0.0578
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAGGGCTACACAGTTACGGT	TGGTATGTACCCTGATCTTGT	50.0	42.9	57.8	55.8	296-298 bp	1.0	55.1
ccsA-ndhD_p2	GGGCTACACAGTTACGGTTC	TGGTATGTACCCTGATCTTGT	55.0	42.9	58.3	55.8	294-296 bp	1.0	54.6
ccsA-ndhD_p3	TAGGGCTACACAGTTACGGT	TGGTATGTACCCTGATCTTGTTC	50.0	43.5	57.8	57.6	296-298 bp	1.0	54.1
ccsA-ndhD_p4	GGGCTACACAGTTACGGTTC	TGGTATGTACCCTGATCTTGTTC	55.0	43.5	58.3	57.6	294-296 bp	1.0	53.6
ccsA-ndhD_p5	GGCTACACAGTTACGGTTCA	TGGTATGTACCCTGATCTTGT	50.0	42.9	57.6	55.8	293-295 bp	1.0	53.3