Hotspot

psbE-petL

LSC · 61332-62181 · 850 bp

Back to Symphytum

Pi 0.0118

MarkerSeek score 51.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 7
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	661-918 bp	1.0	83.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	663-920 bp	1.0	83.4
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GCCGCTAGTAGAAAACCGAA	55.0	50.0	58.2	58.0	656-913 bp	1.0	83.1
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AAGCCGCTAGTAGAAAACCG	55.0	50.0	58.2	58.0	658-915 bp	1.0	83.1
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.4	58.0	654-911 bp	1.0	81.9