Hotspot

psaA-ycf3

LSC · 40716-41446 · 731 bp

Back to Symphytum

Pi 0.014

MarkerSeek score 68.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 10
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTACGGGATCCCTGTCTACC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	57.9	58.0	872-887 bp	1.0	81.4
psaA-ycf3_p2	TTACGGGATCCCTGTCTACC	GGAAACAGGCTATAGCGCTT	55.0	50.0	57.9	58.0	854-869 bp	1.0	81.4
psaA-ycf3_p3	GTTCTTGAAAAATGGCCCGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	918-933 bp	1.0	81.3
psaA-ycf3_p4	GTTCTTGAAAAATGGCCCGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	900-915 bp	1.0	81.3
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	894-909 bp	1.0	81.1