Hotspot

trnS-UGA-psbZ

LSC · 34538-34894 · 357 bp

Back to Symphytum

Pi 0.0252

MarkerSeek score 68.20

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 9
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	477 bp	1.0	79.7
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	479 bp	1.0	79.3
trnS-UGA-psbZ_p3	GGGAGAGAGAGGGATTCGAA	CCAACCATCAGGAGAAGCAA	55.0	50.0	57.9	57.8	549 bp	1.0	79.1
trnS-UGA-psbZ_p4	AAGGGAGAGAGAGGGATTCG	CCAACCATCAGGAGAAGCAA	55.0	50.0	57.9	57.8	551 bp	1.0	79.0
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	497 bp	1.0	78.8