Hotspot

psbI-trnS-GCU

LSC · 7694-7806 · 113 bp

Back to Symphytum

Pi 0.0442

MarkerSeek score 76.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 5
pi: 0.0442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.1	58.0	205 bp	1.0	74.2
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	TAGGAGAGATGGCTGAGTGG	50.0	55.0	57.1	57.9	227 bp	1.0	73.7
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	TAAAGCGGCGGATTGCTAAT	47.6	45.0	57.8	58.0	205 bp	1.0	71.8
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	177 bp	1.0	71.6
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	TAGGAGAGATGGCTGAGTGG	47.6	55.0	57.8	57.9	227 bp	1.0	71.3