Hotspot

rps16-trnQ-UUG

LSC · 5991-6657 · 667 bp

Back to Symphytum

Pi 0.018
MarkerSeek score 58.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
667
variable_sites
12
pi
0.018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCATGTCGTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.9 58.1 752 bp 1.0 80.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 740 bp 1.0 79.6
rps16-trnQ-UUG_p3 ATCATGTCGTTCAAGTCGCA TCTTGAATCGAAATGGGGCG 45.0 50.0 57.9 58.6 813 bp 1.0 75.6
rps16-trnQ-UUG_p4 ATCATGTCGTTCAAGTCGCA TTCGGAGGTTCGAATCCTTC 45.0 50.0 57.9 57.3 756 bp 1.0 75.3
rps16-trnQ-UUG_p5 ATCATGTCGTTCAAGTCGCA TAAGGCAACGGGTTTTGGTC 45.0 50.0 57.9 58.7 782 bp 1.0 75.2