Hotspot

ycf1

SSC · 125920-131379 · 5460 bp

Back to Symphoricarpos

Pi 0

MarkerSeek score 54.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTCTTGAATGAGCCCTTTGA	ACCCCGTAACAAATTGGGAC	42.9	50.0	57.2	58.1	1105 bp	1.0	64.9
ycf1_p2	AGTCTTGAATGAGCCCTTTGA	CCCCGTAACAAATTGGGACT	42.9	50.0	57.2	57.8	1104 bp	1.0	64.0
ycf1_p3	AGTCTTGAATGAGCCCTTTGA	GACCAATCCACCCAATTCCA	42.9	50.0	57.2	57.8	1173 bp	1.0	63.8
ycf1_p4	AGTCTTGAATGAGCCCTTTGA	ATTGGGACTGTGAACCTTGG	42.9	50.0	57.2	57.7	1093 bp	1.0	63.3
ycf1_p5	AGTCTTGAATGAGCCCTTTGA	GTGAACCTTGGTTCTTCCCA	42.9	50.0	57.2	57.6	1084 bp	1.0	62.8