Hotspot

rps16

LSC · 4878-5998 · 1121 bp

Back to Symphoricarpos

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1121
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GTGTTGGATTGGCACTACAT	TGGACAGCTGAACCAATGAC	45.0	50.0	56.0	58.1	1318 bp	1.0	63.3
rps16_p2	AGGTTTAGGTGGGGAAATCA	TGGACAGCTGAACCAATGAC	45.0	50.0	56.0	58.1	1288 bp	1.0	63.2
rps16_p3	GTGTTGGATTGGCACTACAT	GGACAGCTGAACCAATGACT	45.0	50.0	56.0	57.8	1317 bp	1.0	62.6
rps16_p4	AGGTTTAGGTGGGGAAATCA	GGACAGCTGAACCAATGACT	45.0	50.0	56.0	57.8	1287 bp	1.0	62.5
rps16_p5	AGGTTTAGGTGGGGAAATCAA	TGGACAGCTGAACCAATGAC	42.9	50.0	56.8	58.1	1288 bp	1.0	61.6