Hotspot

rpl2

LSC · 87090-88577 · 1488 bp

Back to Symphoricarpos

Pi 0.00806

MarkerSeek score 49.10

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1488
variable_sites: 12
pi: 0.00806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGTAGGAACGTCCACGAATT	CCTACTGGATGCATGCCAAT	50.0	50.0	58.0	58.0	2497-2498 bp	1.0	80.4
rpl2_p2	CGTAGGAACGTCCACGAATT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2546-2547 bp	1.0	80.4
rpl2_p3	ATAATGGTAGATGCCCGGGA	CCTACTGGATGCATGCCAAT	50.0	50.0	58.0	58.0	2147-2148 bp	1.0	80.3
rpl2_p4	CGTAGGAACGTCCACGAATT	ACTTCGGGGAAAGGGTAGAA	50.0	50.0	58.0	58.0	2158-2159 bp	1.0	80.2
rpl2_p5	ATAATGGTAGATGCCCGGGA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2196-2197 bp	1.0	80.2