Hotspot

psbA

LSC · 408-1469 · 1062 bp

Back to Symphoricarpos

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CAATGGCGCCCTCTTGATAA	TGGGAGTCCCTGAGAATTCA	50.0	50.0	58.3	57.7	1183 bp	1.0	74.7
psbA_p2	AATGGCGCCCTCTTGATAAA	TGGGAGTCCCTGAGAATTCA	45.0	50.0	57.6	57.7	1182 bp	1.0	73.8
psbA_p3	ATGGCGCCCTCTTGATAAAA	TGGGAGTCCCTGAGAATTCA	45.0	50.0	57.6	57.7	1181 bp	1.0	73.8
psbA_p4	CAATGGCGCCCTCTTGATAA	AATTCGTGTGCTTGGGAGTC	50.0	50.0	58.3	58.5	1195 bp	1.0	73.6
psbA_p5	GGCGCCCTCTTGATAAAACA	TGGGAGTCCCTGAGAATTCA	50.0	50.0	58.5	57.7	1179 bp	1.0	72.9