Hotspot

matK

LSC · 2023-3537 · 1515 bp

Back to Symphoricarpos

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CCATGCTACATCAACGTTTCAG	CCCCCAACCCTCTACTTTTT	45.5	50.0	58.0	57.0	1672 bp	1.0	56.0
matK_p2	CCATGCTACATCAACGTTTCA	CCCCCAACCCTCTACTTTTT	42.9	50.0	56.9	57.0	1672 bp	1.0	55.3
matK_p3	CCATGCTACATCAACGTTTCAG	CCCTGTTTTGACTGTATCGC	45.5	50.0	58.0	56.8	1712 bp	1.0	54.5
matK_p4	CCATGCTACATCAACGTTTCAG	CCCCAACCCTCTACTTTTTGT	45.5	47.6	58.0	57.8	1671 bp	1.0	54.0
matK_p5	CCATGCTACATCAACGTTTCAG	TGACTGTATCGCACTATGCA	45.5	45.0	58.0	56.8	1704 bp	1.0	54.0