Hotspot

trnN-GUU-ndhF

IRb · 111811-112866 · 1056 bp

Back to Symphoricarpos

Pi 0.00641

MarkerSeek score 65.40

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1056
variable_sites: 6
pi: 0.00641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CCGAACGCTCTACCACTAAG	TGGAAGTGGAAGGAAGGGTA	55.0	50.0	57.8	57.6	1038-1137 bp	1.0	79.5
trnN-GUU-ndhF_p2	CCCCAAGTAGGATTCGAACC	TGGAAGTGGAAGGAAGGGTA	55.0	50.0	57.7	57.6	1078-1177 bp	1.0	78.4
trnN-GUU-ndhF_p3	CGAACGCTCTACCACTAAGC	TGGAAGTGGAAGGAAGGGTA	55.0	50.0	58.5	57.6	1037-1136 bp	1.0	77.4
trnN-GUU-ndhF_p4	CCGAACGCTCTACCACTAAG	GGAAGGGTATTATCCACGCA	55.0	50.0	57.8	57.1	1027-1126 bp	1.0	75.5
trnN-GUU-ndhF_p5	CCGAACGCTCTACCACTAAG	CTGGAAGTGGAAGGAAGGGT	55.0	55.0	57.8	58.9	1039-1138 bp	1.0	75.5