Hotspot

trnS-GCU-trnG-UCC

LSC · 9088-9817 · 730 bp

Back to Symphoricarpos

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	45.0	40.9	58.0	57.7	849 bp	1.0	61.3
trnS-GCU-trnG-UCC_p2	GCTCGTACAACGGATTAGCA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.1	57.7	862 bp	1.0	60.9
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.2	57.7	898 bp	1.0	60.4
trnS-GCU-trnG-UCC_p4	AGCTCGTACAACGGATTAGC	TGAATCAAACCGAAAGATCCCT	50.0	40.9	57.8	57.7	863 bp	1.0	59.9
trnS-GCU-trnG-UCC_p5	TGGAAACGGAAAGAGAGGGA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.3	57.7	903 bp	1.0	59.4