Hotspot

matK-trnK-UUU

LSC · 3538-4258 · 721 bp

Back to Symphoricarpos

Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
721
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TGAGACCCATCTAGCTCGAA AACGGTAGAGTACTCGGCTT 50.0 50.0 57.6 58.2 786 bp 1.0 75.2
matK-trnK-UUU_p2 TGAGACCCATCTAGCTCGAA ACGGTAGAGTACTCGGCTTT 50.0 50.0 57.6 58.2 785 bp 1.0 75.2
matK-trnK-UUU_p3 TTGAGACCCATCTAGCTCGA AACGGTAGAGTACTCGGCTT 50.0 50.0 57.6 58.2 787 bp 1.0 75.2
matK-trnK-UUU_p4 TTGAGACCCATCTAGCTCGA ACGGTAGAGTACTCGGCTTT 50.0 50.0 57.6 58.2 786 bp 1.0 75.2
matK-trnK-UUU_p5 GTTGAGACCCATCTAGCTCG ACGGTAGAGTACTCGGCTTT 55.0 50.0 57.5 58.2 787 bp 1.0 74.6