Hotspot

accD-psaI

LSC · 62103-62796 · 694 bp

Back to Sycopsis

Pi 0.0144

MarkerSeek score 52.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 10
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGGATCTGTAGTAGGCGAG	GGCACCAAAACAGAGGGTAA	55.0	50.0	58.0	58.0	1198-1199 bp	1.0	80.6
accD-psaI_p2	AAGGTTCACAAGCAGCTGAA	GGCACCAAAACAGAGGGTAA	45.0	50.0	57.9	58.0	868-869 bp	1.0	80.6
accD-psaI_p3	GCGAGAAAATCACCCGTTTG	GGCACCAAAACAGAGGGTAA	50.0	50.0	58.0	58.0	1183-1184 bp	1.0	80.5
accD-psaI_p4	TGGGATCTGTAGTAGGCGAG	TCCGCATGCATAAAAGCTCT	55.0	45.0	58.0	58.0	1406 bp	1.0	80.3
accD-psaI_p5	TGGGATCTGTAGTAGGCGAG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.0	58.0	1746 bp	1.0	80.1