Hotspot

ndhK-ndhC

LSC · 53214-53272 · 59 bp

Back to Sycopsis

Pi 0.0169

MarkerSeek score 62.20

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 1
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AGAGAAGCGGCCATAAACTG	TTTATGCATGGCGAAAGGGA	50.0	45.0	58.0	57.9	212 bp	1.0	79.0
ndhK-ndhC_p2	AGAGAAGCGGCCATAAACTG	GGTTCAGTTTATGCATGGCG	50.0	50.0	58.0	57.8	219 bp	1.0	78.6
ndhK-ndhC_p3	AGAGAAGCGGCCATAAACTG	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.0	57.8	213 bp	1.0	78.5
ndhK-ndhC_p4	AGAGAAGCGGCCATAAACTG	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	294 bp	1.0	78.4
ndhK-ndhC_p5	AGAGAAGCGGCCATAAACTG	TTATGCATGGCGAAAGGGAG	50.0	50.0	58.0	58.3	211 bp	1.0	77.6