Hotspot

atpF-atpH

LSC · 14120-14569 · 450 bp

Back to Sycopsis

Pi 0.0111

MarkerSeek score 62.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 5
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	604-617 bp	1.0	74.9
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	CGAGGCGGAGGGAAAAATAC	50.0	55.0	58.0	58.7	609-622 bp	1.0	74.7
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.0	58.7	608-621 bp	1.0	74.7
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	605-618 bp	1.0	74.7
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	606-619 bp	1.0	74.7