Hotspot

trnS-GCU-trnG

LSC · 9261-10100 · 840 bp

Back to Sycopsis

Pi 0.00482

MarkerSeek score 55.40

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 4
pi: 0.00482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	CAATCCGACGCTTTAGTCCA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.0	58.0	953-956 bp	1.0	80.7
trnS-GCU-trnG_p2	AATCCGACGCTTTAGTCCAC	GAATTAAACCGAGGGACCCC	50.0	55.0	58.0	58.0	952-955 bp	1.0	80.7
trnS-GCU-trnG_p3	ACGGAAAGAGAGGGATTCGA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.2	58.0	1007-1010 bp	1.0	79.5
trnS-GCU-trnG_p4	TGGAAACGGAAAGAGAGGGA	GAATTAAACCGAGGGACCCC	50.0	55.0	58.3	58.0	1012-1015 bp	1.0	78.5
trnS-GCU-trnG_p5	AGCAATCCGACGCTTTAGTC	GAATTAAACCGAGGGACCCC	50.0	55.0	58.4	58.0	955-958 bp	1.0	77.9